Human Dentin sialophosphoprotein (DSPP) ELISA Kit | KTE61972

Human Dentin sialophosphoprotein (DSPP) ELISA Kit | KTE61972 | Gentaur UK, US & Europe Distribution

Application: This Human Dentin sialophosphoprotein (DSPP) ELISA Kit employs a two-site sandwich ELISA to quantitate DSPP in samples. An antibody specific for DSPP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDSPP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DSPP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DSPP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Dentin sialophosphoprotein microplate
• Human Dentin sialophosphoprotein standard
• Human Dentin sialophosphoprotein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Dentin sialophosphoprotein (DSPP) ELISA Kit has high sensitivity and excellent specificity for detection of Human DSPP. No significant cross-reactivity or interference between Human DSPP and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Dentin sialophosphoprotein, is the only protein produced uniquely by odontoblasts, the cells that produce tooth dentin. It is a non-collagenous SIBLING protein that is later cleaved into three functional proteins: dentin phosphoprotein (also known as phosphophoryn), taken from the C-terminal end, dentin sialoprotein from the N-terminal end, and dentin glycoprotein from the middle of the molecule. Their distribution in the collagen matrix of the forming dentin suggests these proteins play an important role in the regulation of mineral deposition. Additional evidence for this correlation is phenotypically manifested in patients with mutant forms of dentin sialophosphoprotein. Such patients suffer dental anomalies including type III dentinogenesis imperfecta.

Alternative Names: DSPP; DFNA39; DGI1; DMP3; DPP; DSP; DTDP2; dentin phosphophoryn; dentin phosphoprotein; dentin phosphoryn; dentin sialoprotein

Search name: DSPP; DFNA39; DGI1; DMP3; DPP; DSP; DTDP2; dentin phosphophoryn; dentin phosphoprotein; dentin phosphoryn; dentin sialoprotein

Tag: DSPP