Human Deoxyribonuclease-1-like 1 (DNASE1L1) ELISA Kit | KTE61997

Human Deoxyribonuclease-1-like 1 (DNASE1L1) ELISA Kit | KTE61997 | Gentaur UK, US & Europe Distribution

Application: This Human Deoxyribonuclease-1-like 1 (DNASE1L1) ELISA Kit employs a two-site sandwich ELISA to quantitate DNASE1L1 in samples. An antibody specific for DNASE1L1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNASE1L1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNASE1L1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNASE1L1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Deoxyribonuclease-1-like 1 microplate
• Human Deoxyribonuclease-1-like 1 standard
• Human Deoxyribonuclease-1-like 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Deoxyribonuclease-1-like 1 (DNASE1L1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DNASE1L1. No significant cross-reactivity or interference between Human DNASE1L1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The transcript was present at highest levels in skeletal and cardiac muscle, with lower expression in other tissues. Mutation analysis was performed using DNA samples from 2 unrelated patients with Barth syndrome and from 11 unrelated patients with Emery-Dreifuss muscular dystrophy. No disease-associated mutations were detected in the coding region of the gene; however, Parrish et al. found a novel 190-bp insertion/deletion polymorphism in the 3-prime untranslated region. Translation of the long open reading frame found in the cDNA yielded a putative 302-amino acid protein with 37.6% identity to human DNase I.DNASE1L1 was predicted to contain a signal sequence at the amino terminus, a transmembrane domain near the carboxyl terminus, and a helix-loop-helix domain.

Alternative Names: DNASE1L1; XX-FW83563B9.2; DNAS1L1; DNASEX; DNL1L; XIB; DNase I; lysosomal-like; DNase I-like; muscle-specific; DNase X; OTTHUMP00000031940; OTTHUMP00000032113

Search name: DNASE1L1; XX-FW83563B9.2; DNAS1L1; DNASEX; DNL1L; XIB; DNase I; lysosomal-like; DNase I-like; muscle-specific; DNase X; OTTHUMP00000031940; OTTHUMP00000032113

Tag: DNASE1L1