Human Desmocollin-3 (DSC3) ELISA Kit | KTE61978

Human Desmocollin-3 (DSC3) ELISA Kit | KTE61978 | Gentaur UK, US & Europe Distribution

Application: This Human Desmocollin-3 (DSC3) ELISA Kit employs a two-site sandwich ELISA to quantitate DSC3 in samples. An antibody specific for DSC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDSC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DSC3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DSC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Desmocollin-3 microplate
• Human Desmocollin-3 standard
• Human Desmocollin-3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Desmocollin-3 (DSC3) ELISA Kit has high sensitivity and excellent specificity for detection of Human DSC3. No significant cross-reactivity or interference between Human DSC3 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Desmocollin 3 is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. The protein consisted of a signal peptide of 30 amino acids, a precursor segment of 105 amino acids, and a mature protein of 761 amino acids. Antibodies recognizing the predicted mature adhesion molecule of the protein stained antigens along the cell boundaries of normal human keratinocytes resembling the pattern of desmosome localization.

Alternative Names: DSC3; CDHF3; DSC; DSC1; DSC2; DSC4; HT-CP; desmocollin 4

Search name: DSC3; CDHF3; DSC; DSC1; DSC2; DSC4; HT-CP; desmocollin 4

Tag: DSC3