Human Desmoplakin (DSP) ELISA Kit | KTE62265

Human Desmoplakin (DSP) ELISA Kit | KTE62265 | Gentaur UK, US & Europe Distribution

Application: This Human Desmoplakin (DSP) ELISA Kit employs a two-site sandwich ELISA to quantitate DSP in samples. An antibody specific for DSP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDSP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DSP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DSP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Desmoplakin microplate
• Human Desmoplakin standard
• Human Desmoplakin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Desmoplakin (DSP) ELISA Kit has high sensitivity and excellent specificity for detection of Human DSP. No significant cross-reactivity or interference between Human DSP and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.

Alternative Names: DSP; DPI; DPII; 250/210 kDa paraneoplastic pemphigus antigen; desmoplakin I; desmoplakin

Search name: DSP; DPI; DPII; 250/210 kDa paraneoplastic pemphigus antigen; desmoplakin I; desmoplakin

Tag: DSP