Human Destrin (DSTN) ELISA Kit | AE58869HU

Human Destrin (DSTN) ELISA Kit | AE58869HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: DSTN

Alternative Name: ACTDP; ADF; bA462D18.2; bA462D18.2 (destrin (actin depolymerizing factor ADF) (ACTDP) ) |destrin

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.056 ng/mL

Intra-Assay: ≤5.1%

Inter-Assay: ≤8.9%

Recovery: 0, 89

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate DSTN in samples. An antibody specific for DSTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDSTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DSTN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DSTN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Destrin belongs to the actin-binding proteins ADF/cofilin family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin) . Two transcript variants encoding distinct isoforms have been identified for this gene. In a variety of eukaryotes, destrin regulates actin in the cytoskeleton. Destrin binds actin and is thought to connect it as gelsolin segment-1 does. Furthermore, the binding of actin by destrin and cofilin is regulated negatively by phosphorylation. Destrin can also sever actin filaments. At a pH of 7.3 or higher it can sever the filaments but a lower pH the ability for it to do so is limited.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .