Human Destrin (DSTN) ELISA Kit | KTE62264

Human Destrin (DSTN) ELISA Kit | KTE62264 | Gentaur UK, US & Europe Distribution

Application: This Human Destrin (DSTN) ELISA Kit employs a two-site sandwich ELISA to quantitate DSTN in samples. An antibody specific for DSTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDSTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DSTN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DSTN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Destrin microplate
• Human Destrin standard
• Human Destrin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Destrin (DSTN) ELISA Kit has high sensitivity and excellent specificity for detection of Human DSTN. No significant cross-reactivity or interference between Human DSTN and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Destrin belongs to the actin-binding proteins ADF/cofilin family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin) . Two transcript variants encoding distinct isoforms have been identified for this gene. In a variety of eukaryotes, destrin regulates actin in the cytoskeleton. Destrin binds actin and is thought to connect it as gelsolin segment-1 does. Furthermore, the binding of actin by destrin and cofilin is regulated negatively by phosphorylation. Destrin can also sever actin filaments. At a pH of 7.3 or higher it can sever the filaments but a lower pH the ability for it to do so is limited.

Alternative Names: DSTN; ACTDP; ADF; bA462D18.2; bA462D18.2 (destrin (actin depolymerizing factor ADF) (ACTDP) ) ; destrin

Search name: DSTN; ACTDP; ADF; bA462D18.2; bA462D18.2 (destrin (actin depolymerizing factor ADF) (ACTDP) ) ; destrin

Tag: DSTN