Human Diacylglycerol kinase beta (DGKB) ELISA Kit | KTE62082

Human Diacylglycerol kinase beta (DGKB) ELISA Kit | KTE62082 | Gentaur UK, US & Europe Distribution

Application: This Human Diacylglycerol kinase beta (DGKB) ELISA Kit employs a two-site sandwich ELISA to quantitate DGKB in samples. An antibody specific for DGKB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDGKB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DGKB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DGKB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Diacylglycerol kinase beta microplate
• Human Diacylglycerol kinase beta standard
• Human Diacylglycerol kinase beta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Diacylglycerol kinase beta (DGKB) ELISA Kit has high sensitivity and excellent specificity for detection of Human DGKB. No significant cross-reactivity or interference between Human DGKB and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD) . The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Several alternatively spliced transcript variants encoding different isoforms have been found for DGKB.

Alternative Names: DGKB; DAGK2; DGK; DGK-BETA; KIAA0718; DAG kinase beta; OTTHUMP00000201739; diacylglycerol kinase; beta; diglyceride kinase beta

Search name: DGKB; DAGK2; DGK; DGK-BETA; KIAA0718; DAG kinase beta; OTTHUMP00000201739; diacylglycerol kinase; beta; diglyceride kinase beta

Tag: DGKB