Human Diacylglycerol kinase delta (DGKD) ELISA Kit | KTE62081

Human Diacylglycerol kinase delta (DGKD) ELISA Kit | KTE62081 | Gentaur UK, US & Europe Distribution

Application: This Human Diacylglycerol kinase delta (DGKD) ELISA Kit employs a two-site sandwich ELISA to quantitate DGKD in samples. An antibody specific for DGKD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDGKD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DGKD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DGKD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Diacylglycerol kinase delta microplate
• Human Diacylglycerol kinase delta standard
• Human Diacylglycerol kinase delta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Diacylglycerol kinase delta (DGKD) ELISA Kit has high sensitivity and excellent specificity for detection of Human DGKD. No significant cross-reactivity or interference between Human DGKD and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DGKD encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms.

Alternative Names: DGKD; DGKdelta; FLJ26930; KIAA0145; dgkd-2; DAG kinase delta; diglyceride kinase

Search name: DGKD; DGKdelta; FLJ26930; KIAA0145; dgkd-2; DAG kinase delta; diglyceride kinase

Tag: DGKD