Human Dickkopf-related protein 4 (DKK4) ELISA Kit | KTE62038

Human Dickkopf-related protein 4 (DKK4) ELISA Kit | KTE62038 | Gentaur UK, US & Europe Distribution

Application: This Human Dickkopf-related protein 4 (DKK4) ELISA Kit employs a two-site sandwich ELISA to quantitate DKK4 in samples. An antibody specific for DKK4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDKK4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DKK4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DKK4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Dickkopf-related protein 4 microplate
• Human Dickkopf-related protein 4 standard
• Human Dickkopf-related protein 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Dickkopf-related protein 4 (DKK4) ELISA Kit has high sensitivity and excellent specificity for detection of Human DKK4. No significant cross-reactivity or interference between Human DKK4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. Western blot analysis showed that DKK4 is secreted as 40-, 30- to 32-, and 15- to 17-kD proteins; these proteins were not significantly affected by N-glycanase treatment. N-terminal sequence analysis of the different forms suggested that DKK4 is proteolytically processed, possibly by intracellular furin-type proteases. Functional analysis determined that DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.

Alternative Names: DKK4; DKK-4; MGC129562; MGC129563; dickkopf homolog 4

Search name: DKK4; DKK-4; MGC129562; MGC129563; dickkopf homolog 4

Tag: DKK4