Human Dipeptidase 1 (DPEP1) ELISA Kit | KTE61987

Human Dipeptidase 1 (DPEP1) ELISA Kit | KTE61987 | Gentaur UK, US & Europe Distribution

Application: This Human Dipeptidase 1 (DPEP1) ELISA Kit employs a two-site sandwich ELISA to quantitate DPEP1 in samples. An antibody specific for DPEP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPEP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPEP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPEP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Dipeptidase 1 microplate
• Human Dipeptidase 1 standard
• Human Dipeptidase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Dipeptidase 1 (DPEP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DPEP1. No significant cross-reactivity or interference between Human DPEP1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DPEP1 (EC 3.4.13.11) is a kidney membrane enzyme that hydrolyzes a variety of dipeptides and is implicated in renal metabolism of glutathione and its conjugates, e.g., leukotriene D4 (Kozak and Tate, 1982) . DPEP1 is responsible for hydrolysis of the beta-lactam ring of antibiotics, such as penem and carbapenem (Campbell et al., 1984) . Earlier, beta-lactamase enzymes were thought to occur only in bacteria, where their probable function was in protecting the organisms against the action of beta-lactam antibiotics. These antibiotics exhibit selective toxicity against bacteria but virtual inertness against many eukaryotic cells (Adachi et al., 1990) .

Alternative Names: DPEP1; MBD1; MDP; RDP; OTTHUMP00000175356; dipeptidase 1

Search name: DPEP1; MBD1; MDP; RDP; OTTHUMP00000175356; dipeptidase 1

Tag: DPEP1