Human Dipeptidase 2 (DPEP2) ELISA Kit | KTE61986

Human Dipeptidase 2 (DPEP2) ELISA Kit | KTE61986 | Gentaur UK, US & Europe Distribution

Application: This Human Dipeptidase 2 (DPEP2) ELISA Kit employs a two-site sandwich ELISA to quantitate DPEP2 in samples. An antibody specific for DPEP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPEP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPEP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPEP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Dipeptidase 2 microplate
• Human Dipeptidase 2 standard
• Human Dipeptidase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Dipeptidase 2 (DPEP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DPEP2. No significant cross-reactivity or interference between Human DPEP2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DPEP2 belongs to the membrane-bound dipeptidase (EC 3.4.13.19) family. These enzymes hydrolyze a variety of dipeptides, including leukotriene D4, the beta-lactam ring of some antibiotics, and cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation.The deduced 578-amino acid mouse protein contains several residues conserved among MBDs, including 6 cysteines, 3 histidines, and a glutamic acid that is important for catalytic activity. Mouse and human MBD2 share 80% amino acid identity. Spleen, liver, and skeletal muscle expressed low Mbd2 levels, and no expression was detected in kidney and brain. Phospholipase treatment released Mbd2 from transfected COS-7 cell membranes, indicating that Mbd2 is anchored to the membrane through glycosylphosphatidylinositol modification.

Alternative Names: DPEP2; MBD2; OTTHUMP00000174846

Search name: DPEP2; MBD2; OTTHUMP00000174846

Tag: DPEP2