Human DIS3-like exonuclease 2 (DIS3L2) ELISA Kit | KTE62047

Human DIS3-like exonuclease 2 (DIS3L2) ELISA Kit | KTE62047 | Gentaur UK, US & Europe Distribution

Application: This Human DIS3-like exonuclease 2 (DIS3L2) ELISA Kit employs a two-site sandwich ELISA to quantitate DIS3L2 in samples. An antibody specific for DIS3L2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIS3L2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIS3L2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIS3L2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human DIS3-like exonuclease 2 microplate
• Human DIS3-like exonuclease 2 standard
• Human DIS3-like exonuclease 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human DIS3-like exonuclease 2 (DIS3L2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DIS3L2. No significant cross-reactivity or interference between Human DIS3L2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The exosome is a multisubunit complex composed of several highly conserved subunits, some of which are 3' to 5' exoribonucleases. The complex is involved in a variety of cellular processes and is responsible for degrading unstable mRNAs that contain AU-rich (ARE) elements in their untranslated 3' region. DIS3L2 (DIS3-like exonuclease 2) is an 885 amino acid protein that is thought to function as an exonuclease and may be required for the 3' processing of pre-mRNA into mature mRNA. Defects or chromosomal translocations involving the gene encoding DIS3L2 may be associated with Marfanoid habitus, a genetic disorder characterized by abnormalities in the skeleton, eyes and cardiovascular system. DIS3L2 is expressed as five isoforms due to alternative splicing events.

Alternative Names: DIS3L2; FAM6A; FLJ36974; MGC42174; OTTHUMP00000203808; family with sequence similarity 6; member A

Search name: DIS3L2; FAM6A; FLJ36974; MGC42174; OTTHUMP00000203808; family with sequence similarity 6; member A

Tag: DIS3L2