Human Disks large-associated protein 5 (DLGAP5) ELISA Kit | KTE62021

Human Disks large-associated protein 5 (DLGAP5) ELISA Kit | KTE62021 | Gentaur UK, US & Europe Distribution

Application: This Human Disks large-associated protein 5 (DLGAP5) ELISA Kit employs a two-site sandwich ELISA to quantitate DLGAP5 in samples. An antibody specific for DLGAP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLGAP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLGAP5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLGAP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Disks large-associated protein 5 microplate
• Human Disks large-associated protein 5 standard
• Human Disks large-associated protein 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Disks large-associated protein 5 (DLGAP5) ELISA Kit has high sensitivity and excellent specificity for detection of Human DLGAP5. No significant cross-reactivity or interference between Human DLGAP5 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DLG7 is a kinetochore protein that stabilizes microtubules in vicinity of chromosomes. DLG7 controls spindle dynamics, promotes interkinetochore tension and efficient kinetochore capture. DGL7 is a part of Ran-dependent complex. Stabilization of DGL7 in cell occurs due to phosphorylation by Aurora A kinase. Expression of DGL7 is found in cancer and stem cells. The sequences of the remaining 20 genes were entirely new, and characteristic protein motifs or domains were identified in 32 genes. Other sequence features noted were that the coding sequences of 23 genes were followed by relatively long stretches of 3'-untranslated sequences and that 5 genes contained repetitive sequences in their 3'-untranslated regions. The chromosomal location of these genes has been determined.

Alternative Names: DLGAP5; DLG7; HURP; KIAA0008; Drosophila discs large-1 tumor suppressor-like; discs large homolog 7; discs; large homolog 7; hepatoma up-regulated protein

Search name: DLGAP5; DLG7; HURP; KIAA0008; Drosophila discs large-1 tumor suppressor-like; discs large homolog 7; discs; large homolog 7; hepatoma up-regulated protein

Tag: DLGAP5