Human DNA-directed RNA polymerase I subunit RPA12 (ZNRD1) ELISA Kit | KTE62446

Human DNA-directed RNA polymerase I subunit RPA12 (ZNRD1) ELISA Kit | KTE62446 | Gentaur UK, US & Europe Distribution

Application: This Human DNA-directed RNA polymerase I subunit RPA12 (ZNRD1) ELISA Kit employs a two-site sandwich ELISA to quantitate ZNRD1 in samples. An antibody specific for ZNRD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZNRD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNRD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNRD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human DNA-directed RNA polymerase I subunit RPA12 microplate
• Human DNA-directed RNA polymerase I subunit RPA12 standard
• Human DNA-directed RNA polymerase I subunit RPA12 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human DNA-directed RNA polymerase I subunit RPA12 (ZNRD1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ZNRD1. No significant cross-reactivity or interference between Human ZNRD1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: ZNRD1 encodes a protein with similarity to the Saccharomyces cerevisiae Rpa12p subunit of RNA polymerase I. Alternate splicing of this gene results in two transcript variants encoding the same protein. Additional splice variants have been described, but their full-length sequences have not been determined.Lepourcelet et al. (1996) identified ZNRD1, which they called HTEX6, as 1 of several genes in the HLA-A /HLA-F region of chromosome 6. The deduced protein contains 2 potential zinc-binding motifs and shows significant homology with the A12.2 subunit of yeast polymerase I and mouse Tctex6. Coriton et al. (2000) cloned ZNRD1 from a testis cDNA library. The deduced protein contains 126 amino acids. They also identified splice variants predicted to encode shorter peptides.

Alternative Names: ZNRD1; DAAP-65D20.6; HTEX-6; MGC13376; Rpa12; TEX6; hZR14; tctex-6; OTTHUMP00000161285; OTTHUMP00000161286; RNA polymerase I small specific subunit Rpa12; transcription-associated zinc ribbon protein

Search name: ZNRD1; DAAP-65D20.6; HTEX-6; MGC13376; Rpa12; TEX6; hZR14; tctex-6; OTTHUMP00000161285; OTTHUMP00000161286; RNA polymerase I small specific subunit Rpa12; transcription-associated zinc ribbon protein

Tag: ZNRD1