Human DNA repair protein XRCC2 (XRCC2) ELISA Kit | AE10989HU

Human DNA repair protein XRCC2 (XRCC2) ELISA Kit | AE10989HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: XRCC2

Alternative Name: DKFZp781P0919; DNA repair protein XRCC2|RAD51-like|X-ray repair cross complementing protein 2|X-ray repair; complementing defective; repair in Chinese hamster

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.114 ng/mL

Intra-Assay: ≤4.4%

Inter-Assay: ≤7.3%

Recovery: 1, 03

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate XRCC2 in samples. An antibody specific for XRCC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXRCC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XRCC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XRCC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: XRCC2participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the repair of DNA double-strand breaks by homologous recombination and it functionally complements Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents.Hamster cells deficient in XRCC2 showed a more than 100-fold decrease in homologous recombination induced by double-strand breaks compared with the parental cell line. This defect was corrected to almost wildtype levels by transient transfection with a plasmid expressing XRCC2. The repair defect in XRCC2 mutant cells appeared to be restricted to recombinational repair because nonhomologous end joining was normal.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .