Human DnaJ homolog subfamily C member 13 (DNAJC13) ELISA Kit | KTE62408

Human DnaJ homolog subfamily C member 13 (DNAJC13) ELISA Kit | KTE62408 | Gentaur UK, US & Europe Distribution

Application: This Human DnaJ homolog subfamily C member 13 (DNAJC13) ELISA Kit employs a two-site sandwich ELISA to quantitate DNAJC13 in samples. An antibody specific for DNAJC13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNAJC13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNAJC13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNAJC13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human DnaJ homolog subfamily C member 13 microplate
• Human DnaJ homolog subfamily C member 13 standard
• Human DnaJ homolog subfamily C member 13 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human DnaJ homolog subfamily C member 13 (DNAJC13) ELISA Kit has high sensitivity and excellent specificity for detection of Human DNAJC13. No significant cross-reactivity or interference between Human DNAJC13 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DNAJC13, Contains 1 J domain. DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s and can be categorized into three groups, depending on the presence of other domains. Six DnaJ homologs have been identified in Escherichia coli and 22 in Saccharomyces cerevisiae. Genome-wide analysis has revealed 41 DnaJ/Hsp40 family members in humans.

Alternative Names: DNAJC13; FLJ25863; KIAA0678; RME8; DnaJ domain-containing protein RME-8

Search name: DNAJC13; FLJ25863; KIAA0678; RME8; DnaJ domain-containing protein RME-8

Tag: DNAJC13