Human DNM1L (Dynamin 1 Like Protein) ELISA Kit | ELK9322

Human DNM1L (Dynamin 1 Like Protein) ELISA Kit | ELK9322 | Gentaur UK, US & Europe Distribution

Alternative Names: DRP1; DLP1; DVLP; DYMPLE; HDYNIV; VPS1; Dynamin-related protein 1; Dnm1p/Vps1p-like protein; Dynamin family member proline-rich carboxyl-terminal domain less

Species: Human

Assay Type: Sandwich

Sensitivity: 0.067 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynamin 1 Like Protein (DNM1L) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dynamin 1 Like Protein (DNM1L) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynamin 1 Like Protein (DNM1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dynamin 1 Like Protein (DNM1L) in the samples is then determined by comparing the OD of the samples to the standard curve.