Human Doublesex- and mab-3-related transcription factor A2 (DMRTA2) ELISA Kit | KTE62009

Human Doublesex- and mab-3-related transcription factor A2 (DMRTA2) ELISA Kit | KTE62009 | Gentaur UK, US & Europe Distribution

Application: This Human Doublesex- and mab-3-related transcription factor A2 (DMRTA2) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRTA2 in samples. An antibody specific for DMRTA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRTA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRTA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRTA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Doublesex- and mab-3-related transcription factor A2 microplate
• Human Doublesex- and mab-3-related transcription factor A2 standard
• Human Doublesex- and mab-3-related transcription factor A2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Doublesex- and mab-3-related transcription factor A2 (DMRTA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DMRTA2. No significant cross-reactivity or interference between Human DMRTA2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DMRTA2 is involved in gonadal differentiation. The gene is part of five novel human DM genes that map to three well-defined regions of chromosomes 1, 9, and 19. Several studies have reported QTL in various species and strains of tilapia, regions contributing to sex determination have been identified on linkage groups 1, 3, and 23. Genes contributing to sex-specific mortality have been detected on linkage groups 2, 6, and 23. Mapping of Dax1 joined LG16 and LG21 into a single linkage group. The Amh and Dmrta2 genes were mapped to two distinct regions of LG23. The Amh gene was mapped 5 cM from UNH879 within a QTL region for sex determination and 2 cM from UNH216 within a QTL region for sex-specific mortality. Dmrta2 was mapped 4 cM from UNH848 within another QTL region for sex determination.

Alternative Names: DMRTA2; OTTHUMP00000211162

Search name: DMRTA2; OTTHUMP00000211162

Tag: DMRTA2