Human DPP4 (Dipeptidyl Peptidase IV) ELISA Kit | ELK1595

Human DPP4 (Dipeptidyl Peptidase IV) ELISA Kit | ELK1595 | Gentaur UK, US & Europe Distribution

Alternative Names: CD26; ADA; DPPIV; DPP-IV; ADABP; ADCP2; TP103; Adenosine Deaminase Complexing Protein 2; T-cell activation antigen CD26

Species: Human

Assay Type: Sandwich

Sensitivity: 0.253 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Endocrinology;Cardiovascular biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase IV (DPP4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dipeptidyl Peptidase IV (DPP4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidyl Peptidase IV (DPP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dipeptidyl Peptidase IV (DPP4) in the samples is then determined by comparing the OD of the samples to the standard curve.