Human dSIP (Delta-Sleep Inducing Peptide) ELISA Kit | ELK1946

Human dSIP (Delta-Sleep Inducing Peptide) ELISA Kit | ELK1946 | Gentaur UK, US & Europe Distribution

Alternative Names: DSIPI; TSC22D3; DIP; GILZ; TSC-22R; hDIP; TSC22 Domain Family Protein 3; Glucocorticoid-Induced Leucine Zipper; Delta Sleep Inducing Peptide, Immunoreactor

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 7.15 pg/mL

Standard: 1500 pg/mL

Detection Range: 23.44-1500 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 2h

Research Area: Reproductive science;Neuro science;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Delta-Sleep Inducing Peptide (dSIP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Delta-Sleep Inducing Peptide (dSIP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Delta-Sleep Inducing Peptide (dSIP) in the samples is then determined by comparing the OD of the samples to the standard curve.