Human Dual oxidase 2 (DUOX2) ELISA Kit | KTE61970

Human Dual oxidase 2 (DUOX2) ELISA Kit | KTE61970 | Gentaur UK, US & Europe Distribution

Application: This Human Dual oxidase 2 (DUOX2) ELISA Kit employs a two-site sandwich ELISA to quantitate DUOX2 in samples. An antibody specific for DUOX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDUOX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DUOX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DUOX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Dual oxidase 2 microplate
• Human Dual oxidase 2 standard
• Human Dual oxidase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Dual oxidase 2 (DUOX2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DUOX2. No significant cross-reactivity or interference between Human DUOX2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Dual oxidase is an enzyme that was first identified in the mammalian thyroid gland. In humans, two isoforms are found; hDUOX1 and hDUOX2 (this enzyme) . The protein location is not exclusive to thyroid tissue; hDUOX1 is prominent in airway epithelial cells and hDUOX2 in the salivary glands and gastrointestinal tract.It has been suggested that DUOX2 is the isoform to generate H2O2 utilized by thyroid peroxidase (TPO) for the biosynthesis of thyroid hormones, supported by the discovery of congenital hypothyroidism resultant from an inactivating mutation in the DUOX2 gene. The family currently has seven members including NOX1, NOX2 (formerly known as gp91phox), NOX3, NOX4, NOX5, DUOX1 and DUOX2.This protein is known as a dual oxidase because it has both a peroxidase homology domain and a gp91phox domain.

Alternative Names: DUOX2; LNOX2; NOXEF2; P138-TOX; TDH6; THOX2; NADH/NADPH thyroid oxidase p138-tox; NADPH oxidase/peroxidase DUOX2; NADPH thyroid oxidase 2; dual oxidase-like domains 2; flavoprotein NADPH oxidase; nicotinamide a

Search name: DUOX2; LNOX2; NOXEF2; P138-TOX; TDH6; THOX2; NADH/NADPH thyroid oxidase p138-tox; NADPH oxidase/peroxidase DUOX2; NADPH thyroid oxidase 2; dual oxidase-like domains 2; flavoprotein NADPH oxidase; nicotinamide a

Tag: DUOX2