Human Dual specificity protein phosphatase 5 (DUSP5) ELISA Kit | KTE61968

Human Dual specificity protein phosphatase 5 (DUSP5) ELISA Kit | KTE61968 | Gentaur UK, US & Europe Distribution

Application: This Human Dual specificity protein phosphatase 5 (DUSP5) ELISA Kit employs a two-site sandwich ELISA to quantitate DUSP5 in samples. An antibody specific for DUSP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDUSP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DUSP5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DUSP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Dual specificity protein phosphatase 5 microplate
• Human Dual specificity protein phosphatase 5 standard
• Human Dual specificity protein phosphatase 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Dual specificity protein phosphatase 5 (DUSP5) ELISA Kit has high sensitivity and excellent specificity for detection of Human DUSP5. No significant cross-reactivity or interference between Human DUSP5 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DUSP5 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus.

Alternative Names: DUSP5; DUSP; HVH3; VH1-like phosphatase 3; serine/threonine specific protein phosphatase

Search name: DUSP5; DUSP; HVH3; VH1-like phosphatase 3; serine/threonine specific protein phosphatase

Tag: DUSP5