Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A) ELISA Kit | KTE62263

Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A) ELISA Kit | KTE62263 | Gentaur UK, US & Europe Distribution

Application: This Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A) ELISA Kit employs a two-site sandwich ELISA to quantitate DYRK1A in samples. An antibody specific for DYRK1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDYRK1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DYRK1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DYRK1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A microplate
• Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A standard
• Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A) ELISA Kit has high sensitivity and excellent specificity for detection of Human DYRK1A. No significant cross-reactivity or interference between Human DYRK1A and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DYRK1A encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region.

Alternative Names: DYRK1A; DYRK; DYRK1; HP86; MNB; MNBH; MNB/DYRK protein kinase; OTTHUMP00000109090; dual specificity YAK1-related kinase; mnb protein kinase homolog hp86; protein kinase minibrain homolog; serine/threonine kinase

Search name: DYRK1A; DYRK; DYRK1; HP86; MNB; MNBH; MNB/DYRK protein kinase; OTTHUMP00000109090; dual specificity YAK1-related kinase; mnb protein kinase homolog hp86; protein kinase minibrain homolog; serine/threonine kinase

Tag: DYRK1A