Human Dyn (Big Dynorphin) ELISA Kit | ELK1675

Human Dyn (Big Dynorphin) ELISA Kit | ELK1675 | Gentaur UK, US & Europe Distribution

Alternative Names: B-DYN; Biocytin 13 Dynorphin A 1-13 Amide

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 2.56 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction;Neuro science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Big Dynorphin (Dyn) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Big Dynorphin (Dyn) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Big Dynorphin (Dyn) in the samples is then determined by comparing the OD of the samples to the standard curve.