Human DYNC2H1 (Dynein, Cytoplasmic 2, Heavy Chain 1) ELISA Kit | ELK5354

Human DYNC2H1 (Dynein, Cytoplasmic 2, Heavy Chain 1) ELISA Kit | ELK5354 | Gentaur UK, US & Europe Distribution

Alternative Names: DHC1b; DHC2; DNCH2; DYH1B; hdhc11; Dynein heavy chain isotype 1B; Dynein heavy chain 11; Dynein cytoplasmic heavy chain 2; Cytoplasmic dynein 2 heavy chain

Species: Human

Assay Type: Sandwich

Sensitivity: 0.124 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dynein, Cytoplasmic 2, Heavy Chain 1 (DYNC2H1) in the samples is then determined by comparing the OD of the samples to the standard curve.