Human Dynein heavy chain domain-containing protein 1 (DNHD1) ELISA Kit | KTE61996

Human Dynein heavy chain domain-containing protein 1 (DNHD1) ELISA Kit | KTE61996 | Gentaur UK, US & Europe Distribution

Application: This Human Dynein heavy chain domain-containing protein 1 (DNHD1) ELISA Kit employs a two-site sandwich ELISA to quantitate DNHD1 in samples. An antibody specific for DNHD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNHD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNHD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNHD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Dynein heavy chain domain-containing protein 1 microplate
• Human Dynein heavy chain domain-containing protein 1 standard
• Human Dynein heavy chain domain-containing protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Dynein heavy chain domain-containing protein 1 (DNHD1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DNHD1. No significant cross-reactivity or interference between Human DNHD1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DNHD1 Belongs to the dynein heavy chain family. Dynein was first isolated from Tetrahymena cilia four decades ago. The analysis of the primary structure of the dynein heavy chain and the discovery that many organisms express multiple dynein heavy chains have led to two insights. One, dynein, whose motor domain comprises six AAA modules and two potential mechanical levers, generates movement by a mechanism that is fundamentally different than that which underlies the motion of myosin and kinesin. And two, organisms with cilia or flagella express approximately 14 different dynein heavy chain genes, each gene encodes a distinct dynein protein isoform, and each isoform appears to be functionally specialized. Sequence comparisons demonstrate that functionally equivalent isoforms of dynein heavy chains are well conserved across species.

Alternative Names: DNHD1; C11orf47; CCDC35; DHCD1; DKFZp434G0812; DKFZp686J0796; DKFZp686N1238; DNHD1L; FLJ00251; FLJ32752; FLJ35709; FLJ39625; FLJ43897; FLJ46184; MGC133191; DNHD1 variant protein; coiled-coil domain containi

Search name: DNHD1; C11orf47; CCDC35; DHCD1; DKFZp434G0812; DKFZp686J0796; DKFZp686N1238; DNHD1L; FLJ00251; FLJ32752; FLJ35709; FLJ39625; FLJ43897; FLJ46184; MGC133191; DNHD1 variant protein; coiled-coil domain containi

Tag: DNHD1