Human DYSF (Dysferlin) ELISA Kit | ELK3572

Human DYSF (Dysferlin) ELISA Kit | ELK3572 | Gentaur UK, US & Europe Distribution

Alternative Names: FER1L1; LGMD2B; Dysferlin, Limb Girdle Muscular Dystrophy 2B (Autosomal Recessive) ; Fer-1-like protein 1; Dystrophy-associated fer-1-like protein

Species: Human

Assay Type: Sandwich

Sensitivity: 12.5 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Immune molecule;Neuro science;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dysferlin (DYSF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dysferlin (DYSF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dysferlin (DYSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dysferlin (DYSF) in the samples is then determined by comparing the OD of the samples to the standard curve.