Human Dystrobrevin beta (DTNB) ELISA Kit | KTE61971

Human Dystrobrevin beta (DTNB) ELISA Kit | KTE61971 | Gentaur UK, US & Europe Distribution

Application: This Human Dystrobrevin beta (DTNB) ELISA Kit employs a two-site sandwich ELISA to quantitate DTNB in samples. An antibody specific for DTNB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDTNB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DTNB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DTNB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Dystrobrevin beta microplate
• Human Dystrobrevin beta standard
• Human Dystrobrevin beta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Dystrobrevin beta (DTNB) ELISA Kit has high sensitivity and excellent specificity for detection of Human DTNB. No significant cross-reactivity or interference between Human DTNB and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Dystrobrevin beta, a component of the dystrophin-associated protein complex (DPC) . The DPC consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and dystrobrevin alpha and beta. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Dystrobrevin beta is thought to interact with syntrophin and the DP71 short form of dystrophin. Alternatively spliced transcript variants encoding different isoforms have been identified.The sarcoglycan complex is composed of 4 transmembrane glycoproteins: alpha-, beta-, gamma-, and delta-sarcoglycan, and a 25-kD protein 25DAP. Mutations in all 4 sarcoglycan genes have been found in patients with different forms of limb-girdle muscular dystrophy.

Alternative Names: DTNB; MGC17163; MGC57126; OTTHUMP00000201258

Search name: DTNB; MGC17163; MGC57126; OTTHUMP00000201258

Tag: DTNB