Human E2F3 (E2F Transcription Factor 3) ELISA Kit | ELK4530

Human E2F3 (E2F Transcription Factor 3) ELISA Kit | ELK4530 | Gentaur UK, US & Europe Distribution

Alternative Names: E2F 3; E2F transcription factor 3; E2F-3

Species: Human

Assay Type: Sandwich

Sensitivity: 0.072 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E2F Transcription Factor 3 (E2F3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E2F Transcription Factor 3 (E2F3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E2F Transcription Factor 3 (E2F3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2F Transcription Factor 3 (E2F3) in the samples is then determined by comparing the OD of the samples to the standard curve.