Human E3 ubiquitin-protein ligase TRIM68 (TRIM68) ELISA Kit | KTE62549

Human E3 ubiquitin-protein ligase TRIM68 (TRIM68) ELISA Kit | KTE62549 | Gentaur UK, US & Europe Distribution

Application: This Human E3 ubiquitin-protein ligase TRIM68 (TRIM68) ELISA Kit employs a two-site sandwich ELISA to quantitate TRIM68 in samples. An antibody specific for TRIM68 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRIM68 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRIM68 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRIM68 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human E3 ubiquitin-protein ligase TRIM68 microplate
• Human E3 ubiquitin-protein ligase TRIM68 standard
• Human E3 ubiquitin-protein ligase TRIM68 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human E3 ubiquitin-protein ligase TRIM68 (TRIM68) ELISA Kit has high sensitivity and excellent specificity for detection of Human TRIM68. No significant cross-reactivity or interference between Human TRIM68 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: TRIM68 contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene is expressed in many cancer cell lines. Its expression in normal tissues, however, was found to be restricted to prostate. This gene was also found to be differentially expressed in androgen-dependent versus androgen-independent prostate cancer cells.The deduced 485-amino acid protein has a calculated molecular mass of 56 kD. It has a RING finger domain, followed by a B box and a coiled-coil region. The deduced protein contains an N-terminal cys-his cluster, followed by a bipartite nuclear localization signal, a coiled-coil leucine zipper-like domain, and a C-terminal RET finger protein (TRIM27) -like domain.

Alternative Names: TRIM68; FLJ10369; GC109; MGC126176; RNF137; SS-56; SS56; Ro/SSA1 related protein; SSA protein SS-56; ring finger protein 137

Search name: TRIM68; FLJ10369; GC109; MGC126176; RNF137; SS-56; SS56; Ro/SSA1 related protein; SSA protein SS-56; ring finger protein 137

Tag: TRIM68