Human E3 ubiquitin-protein ligase UBR1 (UBR1) ELISA Kit | AE12391HU

Human E3 ubiquitin-protein ligase UBR1 (UBR1) ELISA Kit | AE12391HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: UBR1

Alternative Name: JBS; MGC142065; MGC142067; E3a ligase|ubiquitin ligase E3 alpha-I|ubiquitin-protein ligase E3-alpha

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.055 ng/mL

Intra-Assay: ≤6.3%

Inter-Assay: ≤10.9%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate UBR1 in samples. An antibody specific for UBR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The mouse Ubr1 protein is a 1, 757-residue, 200-kD protein that contains regions with sequence similarity to the 225-kD UBR1 protein of S. cerevisiae. There are apparent UBR1 homologs in other eukaryotes, thus defining a distinct family of proteins, the UBR family. The residues essential for substrate recognition by the yeast UBR1 protein are conserved in the mouse Ubr1 protein. Regions of similarity among the UBR family members include a putative zinc finger and RING-H2 finger, another zinc-binding domain. Northern blot analysis detected ubiquitous expression in human and adult mouse tissues, with highest levels in skeletal muscle and heart. In mouse embryos, in situ hybridization showed that Ubr1 expression is highest in the branchial arches and in the tail and limb buds.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .