Human E3 ubiquitin-protein ligase UBR4 (UBR4) ELISA Kit | AE12385HU

Human E3 ubiquitin-protein ligase UBR4 (UBR4) ELISA Kit | AE12385HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: UBR4

Alternative Name: RP5-1126H10.1; FLJ41863; KIAA0462; KIAA1307; RBAF600; ZUBR1; p600; retinoblastoma-associated factor 600|retinoblastoma-associated factor 600-like protein|zinc finger; UBR1 type 1

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.064 ng/mL

Intra-Assay: ≤6.4%

Inter-Assay: ≤11.5%

Recovery: 0, 99

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate UBR4 in samples. An antibody specific for UBR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBR4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: RBAF600 associates with RB in the nucleus and with Ca (2+) -bound calmodulin in the cytoplasm.p600 and RB appears to act as a chromatin scaffold, and in the cytoplasm p600 and clathrin form a meshwork structure which could contribute to cytoskeletal organization and membrane morphogenesis. Reduced expression of p600 with interfering RNA abrogated integrin-mediated ruffled membrane formation and prevented activation of integrin-mediated survival pathways. The deduced 5, 183-amino acid protein has a calculated molecular mass of 573.5 kD. Immunostaining of human foreskin fibroblasts localized p600 in a continuous meshwork pattern from the nucleus to the cytoplasm, passing through the nuclear envelope. In the cytoplasm, p600 was concentrated at the leading edge of membrane structures.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .