Human EAAT3 (Excitatory Amino Acid Transporter 3) ELISA Kit | ELK4429

Human EAAT3 (Excitatory Amino Acid Transporter 3) ELISA Kit | ELK4429 | Gentaur UK, US & Europe Distribution

Alternative Names: SLC1A1; EAAC1; Solute Carrier 1 Member 1; Sodium-dependent glutamate/aspartate transporter 3; Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter

Species: Human

Assay Type: Sandwich

Sensitivity: 0.117 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excitatory Amino Acid Transporter 3 (EAAT3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Excitatory Amino Acid Transporter 3 (EAAT3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Excitatory Amino Acid Transporter 3 (EAAT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Excitatory Amino Acid Transporter 3 (EAAT3) in the samples is then determined by comparing the OD of the samples to the standard curve.