Human EGF (Epidermal Growth Factor) ELISA Kit | ELK1266

Human EGF (Epidermal Growth Factor) ELISA Kit | ELK1266 | Gentaur UK, US & Europe Distribution

Alternative Names: URG; Beta-Urogastrone; Pro-epidermal growth factor

Species: Human

Assay Type: Sandwich

Sensitivity: 1.5 pg/mL

Standard: 250 pg/mL

Detection Range: 3.91-250 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epidermal Growth Factor (EGF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Epidermal Growth Factor (EGF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epidermal Growth Factor (EGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epidermal Growth Factor (EGF) in the samples is then determined by comparing the OD of the samples to the standard curve.