Human EGFL6 (EGF Like Domain Protein, Multiple 6) ELISA Kit | ELK6160

Human EGFL6 (EGF Like Domain Protein, Multiple 6) ELISA Kit | ELK6160 | Gentaur UK, US & Europe Distribution

Alternative Names: MAEG; MAM And EGF Domain Containing; Epidermal growth factor-like protein 6

Species: Human

Assay Type: Sandwich

Sensitivity: 35.95 pg/mL

Standard: 6000 pg/mL

Detection Range: 93.75-6000 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Domain Protein, Multiple 6 (EGFL6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to EGF Like Domain Protein, Multiple 6 (EGFL6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Domain Protein, Multiple 6 (EGFL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EGF Like Domain Protein, Multiple 6 (EGFL6) in the samples is then determined by comparing the OD of the samples to the standard curve.