Human Electrogenic sodium bicarbonate cotransporter 4 (SLC4A5) ELISA Kit | KTE60599

Human Electrogenic sodium bicarbonate cotransporter 4 (SLC4A5) ELISA Kit | KTE60599 | Gentaur UK, US & Europe Distribution

Application: This Human Electrogenic sodium bicarbonate cotransporter 4 (SLC4A5) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC4A5 in samples. An antibody specific for SLC4A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC4A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC4A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC4A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Electrogenic sodium bicarbonate cotransporter 4 microplate
• Human Electrogenic sodium bicarbonate cotransporter 4 standard
• Human Electrogenic sodium bicarbonate cotransporter 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Electrogenic sodium bicarbonate cotransporter 4 (SLC4A5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC4A5. No significant cross-reactivity or interference between Human SLC4A5 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: SLC4A5 is a member of the sodium bicarbonate cotransporter (NBC) family, part of the bicarbonate transporter superfamily. Sodium bicarbonate cotransporters are involved in intracellular pH regulation and electroneural or electrogenic sodium bicarbonate transport. This protein is thought to be an integral membrane protein. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.Highest expression observed in liver, spleen and testis; moderate expression in the choroid plexus, hippocampus, cerebrum and cerebellum of brain, and in kidney cortex and kidney medulla. Also observed in heart, pancreas, muscle, lung, placenta, stomach and small intestine.

Alternative Names: SLC4A5; MGC129662; NBC4; OTTHUMP00000115292; sodium bicarbonate transporter 4

Search name: SLC4A5; MGC129662; NBC4; OTTHUMP00000115292; sodium bicarbonate transporter 4

Tag: SLC4A5