Human Endothelin-converting enzyme 2 (ECE2) ELISA Kit | KTE61958

Human Endothelin-converting enzyme 2 (ECE2) ELISA Kit | KTE61958 | Gentaur UK, US & Europe Distribution

Application: This Human Endothelin-converting enzyme 2 (ECE2) ELISA Kit employs a two-site sandwich ELISA to quantitate ECE2 in samples. An antibody specific for ECE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyECE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ECE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ECE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Endothelin-converting enzyme 2 microplate
• Human Endothelin-converting enzyme 2 standard
• Human Endothelin-converting enzyme 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Endothelin-converting enzyme 2 (ECE2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ECE2. No significant cross-reactivity or interference between Human ECE2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Endothelin-converting enzymes, such as ECE2 are type II metalloproteases that generate functionally pleiotropic members of the endothelin vasoactive peptide family.The deduced protein contains 765 amino acids. ECE2 had an apparent molecular mass of 81 kD by SDS-PAGE.The ECE2A variant contains exons 1A and 2A and encodes a deduced 787-amino acid protein with a calculated molecular mass of 89.1 kD. ECE2A has an intracytoplasmic tail, a transmembrane domain, and a large extracellular catalytic domain with a zinc-binding motif conserved in Zn (2+) metalloproteases. It has 9 N-glycosylation sites. The ECE2B variant contains exons 1B and 2B and encodes a deduced 682-amino acid protein with a calculated molecular mass of 86.4 kD. ECE2B differs from ECE2A only in the intracytoplasmic tail.

Alternative Names: ECE2; hCG_2022032; KIAA0604; MGC17664; MGC2408; MGC78487;

Search name: ECE2; hCG_2022032; KIAA0604; MGC17664; MGC2408; MGC78487;

Tag: ECE2