Human EPHA1 (Ephrin Type A Receptor 1) ELISA Kit | ELK1899

Human EPHA1 (Ephrin Type A Receptor 1) ELISA Kit | ELK1899 | Gentaur UK, US & Europe Distribution

Alternative Names: EPH; EPHT; EPHT1; EPH Receptor A1; EPH tyrosine kinase 1; Erythropoietin-producing hepatoma receptor; Tyrosine-protein kinase receptor EPH

Species: Human

Assay Type: Sandwich

Sensitivity: 0.124 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity;Neuro science;Developmental science;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin Type A Receptor 1 (EPHA1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ephrin Type A Receptor 1 (EPHA1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin Type A Receptor 1 (EPHA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ephrin Type A Receptor 1 (EPHA1) in the samples is then determined by comparing the OD of the samples to the standard curve.