Human EPHA10 (Ephrin Type A Receptor 10) ELISA Kit | ELK3633

Human EPHA10 (Ephrin Type A Receptor 10) ELISA Kit | ELK3633 | Gentaur UK, US & Europe Distribution

Alternative Names: EPH receptor A10; EPHA 10; Epha10; EphA10s protein; EPHAA_HUMAN; Ephrin type A receptor 10; Ephrin type-A receptor 10; FLJ16103; FLJ33655; MGC43817

Species: Human

Assay Type: Sandwich

Sensitivity: 0.109 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin Type A Receptor 10 (EPHA10) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ephrin Type A Receptor 10 (EPHA10) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin Type A Receptor 10 (EPHA10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ephrin Type A Receptor 10 (EPHA10) in the samples is then determined by comparing the OD of the samples to the standard curve.