Human Epidermal growth factor Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit | KTE61943

Human Epidermal growth factor Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit | KTE61943 | Gentaur UK, US & Europe Distribution

Application: This Human Epidermal growth factor Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit employs a two-site sandwich ELISA to quantitate EGFL7 in samples. An antibody specific for EGFL7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEGFL7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFL7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFL7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Epidermal growth factor Like Domain Protein, Multiple 7 microplate
• Human Epidermal growth factor Like Domain Protein, Multiple 7 standard
• Human Epidermal growth factor Like Domain Protein, Multiple 7 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Epidermal growth factor Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit has high sensitivity and excellent specificity for detection of Human EGFL7. No significant cross-reactivity or interference between Human EGFL7 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The deduced 275-amino acid protein has a calculated molecular mass of 29.8 kD. The mouse and human EGFL7 proteins both contain an N-terminal cleavable signal peptide followed by 2 epidermal growth factor (EGF) -like domains, and they share 78% amino acid identity. Northern blot analysis detected a transcript of about 1.6 kb only in mouse heart, lung, and kidney. In situ hybridization showed mouse Egfl7 expressed at embryonic day 7.5 exclusively in the primitive blood islands where the first endothelial cells differentiate, and later in endothelial cells of a wide range of tissues. Fluorescence-labeled Egfl7 was expressed in the endoplasmic reticulum of transfected mouse fibroblasts, and it was secreted into the culture medium.

Alternative Names: EGFL7; MGC111117; RP11-251M1.2; VE-STATIN; ZNEU1; NEU1 protein; OTTHUMP00000022597

Search name: EGFL7; MGC111117; RP11-251M1.2; VE-STATIN; ZNEU1; NEU1 protein; OTTHUMP00000022597

Tag: EGFL7