Human Epidermal growth factor-like protein 6 (EGFL6) ELISA Kit | KTE61944

Human Epidermal growth factor-like protein 6 (EGFL6) ELISA Kit | KTE61944 | Gentaur UK, US & Europe Distribution

Application: This Human Epidermal growth factor-like protein 6 (EGFL6) ELISA Kit employs a two-site sandwich ELISA to quantitate EGFL6 in samples. An antibody specific for EGFL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEGFL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFL6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Epidermal growth factor-like protein 6 microplate
• Human Epidermal growth factor-like protein 6 standard
• Human Epidermal growth factor-like protein 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Epidermal growth factor-like protein 6 (EGFL6) ELISA Kit has high sensitivity and excellent specificity for detection of Human EGFL6. No significant cross-reactivity or interference between Human EGFL6 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: EGFL6 encodes a member of the epidermal growth factor (EGF) repeat superfamily.The predicted 553-amino acid EGFL6 protein has a putative N-terminal signal peptide, which suggests that it is secreted; an EGF repeat region containing 4 complete EGF-like repeats and 1 partial EGF-like repeat; an integrin association motif (RGD) ; 2 potential N-glycosylation sites; and a potential tyrosine phosphorylation site. Northern blot analysis of a variety of normal human tissues detected an approximately 2.4-kb EGFL6 transcript only in placenta. Among the cancer tissues tested, EGFL6 expression was found only in meningioma tumors. Screening-by-hybridization analysis of various cDNA libraries indicated EGFL6 expression in lung tumor, fetal lung, fetal skin, fetal umbilical cord, fetal liver/spleen, and placenta, but not in normal adult tissues, including lung.

Alternative Names: EGFL6; DKFZp564P2063; MAEG; W80; EGF repeat-containing protein 6; MAM and EGF domain containing; epidermal growth factor-like protein 6

Search name: EGFL6; DKFZp564P2063; MAEG; W80; EGF repeat-containing protein 6; MAM and EGF domain containing; epidermal growth factor-like protein 6

Tag: EGFL6