Human Epididymal-specific lipocalin-6 (LCN6) ELISA Kit | KTE62378

Human Epididymal-specific lipocalin-6 (LCN6) ELISA Kit | KTE62378 | Gentaur UK, US & Europe Distribution

Application: This Human Epididymal-specific lipocalin-6 (LCN6) ELISA Kit employs a two-site sandwich ELISA to quantitate LCN6 in samples. An antibody specific for LCN6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLCN6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LCN6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCN6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Epididymal-specific lipocalin-6 microplate
• Human Epididymal-specific lipocalin-6 standard
• Human Epididymal-specific lipocalin-6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Epididymal-specific lipocalin-6 (LCN6) ELISA Kit has high sensitivity and excellent specificity for detection of Human LCN6. No significant cross-reactivity or interference between Human LCN6 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Using database analysis and cDNA sequencing from a human caput library, Hamil et al. (2003) identified LCN6. The 163-amino acid LCN6 protein contains a predicted 20-amino acid signal peptide.human LCN6 is 93% identical to rhesus monkey Lcn6 and lacks a second, C-terminal cysteine found in many lipocalins. The LCN6 protein contains multiple phosphorylation sites, and structural prediction programs showed that LCN6 displays the characteristic lipocalin 3-dimensional structure. Northern blot analysis revealed robust expression of a 1-kb LCN6 transcript in epididymis. Using immunohistochemistry, strong expression of LCN6 protein in the caput epithelium and lumen; immunofluorescence of human spermatozoa showed strong LCN6 expression in large patches in the postacrosomal region of the head.

Alternative Names: LCN6; RP11-216L13.2; LCN5; UNQ643; hLcn5; epididymal-specific lipocalin LCN6; lipocalin 5

Search name: LCN6; RP11-216L13.2; LCN5; UNQ643; hLcn5; epididymal-specific lipocalin LCN6; lipocalin 5

Tag: LCN6