Human EPO (Erythropoietin) ELISA Kit | ELK1011

Human EPO (Erythropoietin) ELISA Kit | ELK1011 | Gentaur UK, US & Europe Distribution

Alternative Names: EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin

Species: Human

Assay Type: Sandwich

Sensitivity: 11.5 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Endocrinology;Hematology;Hormone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erythropoietin (EPO) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Erythropoietin (EPO) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Erythropoietin (EPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Erythropoietin (EPO) in the samples is then determined by comparing the OD of the samples to the standard curve.