Human ErbB4 (V-Erb A Erythroblastic Leukemia Viral Oncogene Homolog 4) ELISA Kit | ELK4703

Human ErbB4 (V-Erb A Erythroblastic Leukemia Viral Oncogene Homolog 4) ELISA Kit | ELK4703 | Gentaur UK, US & Europe Distribution

Alternative Names: HER4; P180erbB4; Receptor Tyrosine-Protein Kinase ErbB-4; Proto-oncogene-like protein c-ErbB-4; Tyrosine kinase-type cell surface receptor HER4

Species: Human

Assay Type: Sandwich

Sensitivity: 0.062 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Erb A Erythroblastic Leukemia Viral Oncogene Homolog 4 (ErbB4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to V-Erb A Erythroblastic Leukemia Viral Oncogene Homolog 4 (ErbB4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Erb A Erythroblastic Leukemia Viral Oncogene Homolog 4 (ErbB4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Erb A Erythroblastic Leukemia Viral Oncogene Homolog 4 (ErbB4) in the samples is then determined by comparing the OD of the samples to the standard curve.