Human Extracellular glycoprotein lacritin (LACRT) ELISA Kit | KTE61868

Human Extracellular glycoprotein lacritin (LACRT) ELISA Kit | KTE61868 | Gentaur UK, US & Europe Distribution

Application: This Human Extracellular glycoprotein lacritin (LACRT) ELISA Kit employs a two-site sandwich ELISA to quantitate LACRT in samples. An antibody specific for LACRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLACRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LACRT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LACRT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Extracellular glycoprotein lacritin microplate
• Human Extracellular glycoprotein lacritin standard
• Human Extracellular glycoprotein lacritin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Extracellular glycoprotein lacritin (LACRT) ELISA Kit has high sensitivity and excellent specificity for detection of Human LACRT. No significant cross-reactivity or interference between Human LACRT and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Lacritin is a 12.3 kDa glycoprotein encoded in humans by the LACRT gene. Lacritin is a secreted protein found in tears and saliva. Lacritin also promotes tear secretion and proliferation of some epithelial cells. Lacritin is thus a prosecretory mitogen. Most lacritin is produced by the lacrimal gland. Some lacritin is produced by the meibomian gland, and also by epithelial cells of the conjunctiva and cornea. Together these epithelia comprise much of the lacrimal functional unit (LFU) . Dry eye is the most common disease of the LFU. Preliminary studies with small trials suggest that lacritin may be differentially downregulated in dry eye. Topical lacritin promotes tearing in rabbit preclinical studies. Lacritin cell targeting is dependent on the cell surface heparan sulfate proteoglycan syndecan-1 (SDC1) .

Alternative Names: LACRT; MGC71934;

Search name: LACRT; MGC71934;

Tag: LACRT