Human F13 (Coagulation Factor XIII) ELISA Kit | ELK1767

Human F13 (Coagulation Factor XIII) ELISA Kit | ELK1767 | Gentaur UK, US & Europe Distribution

Alternative Names: FXIII; Fibrin Stabilizing Factor

Species: Human

Assay Type: Sandwich

Sensitivity: 8 pg/mL

Standard: 1600 pg/mL

Detection Range: 25-1600 pg/mL

Sample Type: plasma

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor XIII (F13) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Coagulation Factor XIII (F13) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor XIII (F13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor XIII (F13) in the samples is then determined by comparing the OD of the samples to the standard curve.