Human F13B (Coagulation Factor XIII B Polypeptide) ELISA Kit | ELK4996

Human F13B (Coagulation Factor XIII B Polypeptide) ELISA Kit | ELK4996 | Gentaur UK, US & Europe Distribution

Alternative Names: F13-B; FXIIIB; Fibrin-stabilizing factor B subunit; Protein-glutamine gamma-glutamyltransferase B chain; Transglutaminase B chain

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 285.1 ng/mL

Standard: 50000 ng/mL

Detection Range: 781.25-50000 ng/mL

Sample Type: plasma

Assay Lenght: 2h

Research Area: Signal transduction;Metabolic pathway;Hematology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Coagulation Factor XIII B Polypeptide (F13B) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Coagulation Factor XIII B Polypeptide (F13B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor XIII B Polypeptide (F13B) in the samples is then determined by comparing the OD of the samples to the standard curve.