Human FAM5C (Family With Sequence Similarity 5, Member C) ELISA Kit | ELK6170

Human FAM5C (Family With Sequence Similarity 5, Member C) ELISA Kit | ELK6170 | Gentaur UK, US & Europe Distribution

Alternative Names: DBCCR1L; DBCCR1L1; DBCCR1-like protein 1; BMP/retinoic acid-inducible neural-specific protein 3

Species: Human

Assay Type: Sandwich

Sensitivity: 0.116 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Family With Sequence Similarity 5, Member C (FAM5C) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Family With Sequence Similarity 5, Member C (FAM5C) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Family With Sequence Similarity 5, Member C (FAM5C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Family With Sequence Similarity 5, Member C (FAM5C) in the samples is then determined by comparing the OD of the samples to the standard curve.