Human Far upstream element-binding protein 2 (KHSRP) ELISA Kit | KTE61922

Human Far upstream element-binding protein 2 (KHSRP) ELISA Kit | KTE61922 | Gentaur UK, US & Europe Distribution

Application: This Human Far upstream element-binding protein 2 (KHSRP) ELISA Kit employs a two-site sandwich ELISA to quantitate KHSRP in samples. An antibody specific for KHSRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKHSRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KHSRP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KHSRP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Far upstream element-binding protein 2 microplate
• Human Far upstream element-binding protein 2 standard
• Human Far upstream element-binding protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Far upstream element-binding protein 2 (KHSRP) ELISA Kit has high sensitivity and excellent specificity for detection of Human KHSRP. No significant cross-reactivity or interference between Human KHSRP and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization. The pre-mRNA of the protooncogene SRC contains an 18-nucleotide exon, N1, that is spliced into the mRNA in neuronal cells but is excluded in nonneuronal cells. N1 exon inclusion in neurons is under the positive control of an intronic regulatory sequence called the downstream control sequence (DCS) .The predicted 711-amino acid protein contains a proline/glycine-rich N terminus and a C-terminal domain that is rich in proline, glycine, alanine, and glutamine. The central region contains 4 tandemly repeated KH domains, leading the authors to rename the protein 'KH-type splicing regulatory protein' (KSRP) .

Alternative Names: KHSRP; FBP2; FUBP2; KSRP; MGC99676; FUSE binding protein 2; far upstream element-binding protein 2

Search name: KHSRP; FBP2; FUBP2; KSRP; MGC99676; FUSE binding protein 2; far upstream element-binding protein 2

Tag: KHSRP