Human FCN2 (Ficolin 2) ELISA Kit | ELK2672

Human FCN2 (Ficolin 2) ELISA Kit | ELK2672 | Gentaur UK, US & Europe Distribution

Alternative Names: EBP37; FCNL; P35; Hucolin; 37 kDa elastin-binding protein; Collagen/Fibrinogen Domain Containing Lectin; Ficolin B; Serum Lectin p35; L-Ficolin

Species: Human

Assay Type: Sandwich

Sensitivity: 0.35 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Hematology;Cardiovascular biology;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ficolin 2 (FCN2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ficolin 2 (FCN2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ficolin 2 (FCN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ficolin 2 (FCN2) in the samples is then determined by comparing the OD of the samples to the standard curve.